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After transformation, 945 L of liquid LB medium (without ampicillin) was added to the cells, which were then incubated at 37 C with shaking at 200 rpm for 1 h

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Repeated dosing study of resmetirom and semaglutide efficacy in WD-fed MC4R-KO mice WD-fed MC4R-KO mice were divided into the following three groups: vehicle (0.5% methyl cellulose [MC], p.o.) ( n = 8), resmetirom (5 mg/kg, p.o.) ( n = 8), semaglutide (0.1 mg/kg s.c.) ( n = 4)

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